αs1 casein Search Results


bovine αs1-casein reference standard  (Carl Roth GmbH)
90
Carl Roth GmbH bovine αs1-casein reference standard
Bovine αs1 Casein Reference Standard, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bovine αs1-casein reference standard/product/Carl Roth GmbH
Average 90 stars, based on 1 article reviews
bovine αs1-casein reference standard - by Bioz Stars, 2026-03
90/100 stars
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rabbit anti-human αs1-casein (csn1s1)  (ModiQuest)
90
ModiQuest rabbit anti-human αs1-casein (csn1s1)
The environment of the fusion sites is given as sequences. The eight amino acids at the N-terminus and the two amino acids at the C-terminus that were added to <t>CSN1S1</t> due to the cloning procedure are shown in italics. Restriction sites used for cloning are underlined. SP, signal peptide.
Rabbit Anti Human αs1 Casein (Csn1s1), supplied by ModiQuest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-human αs1-casein (csn1s1)/product/ModiQuest
Average 90 stars, based on 1 article reviews
rabbit anti-human αs1-casein (csn1s1) - by Bioz Stars, 2026-03
90/100 stars
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festivo fermented low-fat hard cheese αs1-casein f (1–9), αs1-casein f (1–7), αs1-casein f (1–6)  (Agri-Food Laboratories)
90
Agri-Food Laboratories festivo fermented low-fat hard cheese αs1-casein f (1–9), αs1-casein f (1–7), αs1-casein f (1–6)
The environment of the fusion sites is given as sequences. The eight amino acids at the N-terminus and the two amino acids at the C-terminus that were added to <t>CSN1S1</t> due to the cloning procedure are shown in italics. Restriction sites used for cloning are underlined. SP, signal peptide.
Festivo Fermented Low Fat Hard Cheese αs1 Casein F (1–9), αs1 Casein F (1–7), αs1 Casein F (1–6), supplied by Agri-Food Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/festivo fermented low-fat hard cheese αs1-casein f (1–9), αs1-casein f (1–7), αs1-casein f (1–6)/product/Agri-Food Laboratories
Average 90 stars, based on 1 article reviews
festivo fermented low-fat hard cheese αs1-casein f (1–9), αs1-casein f (1–7), αs1-casein f (1–6) - by Bioz Stars, 2026-03
90/100 stars
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small interference rna si-αs1-casein  (Shanghai GenePharma)
90
Shanghai GenePharma small interference rna si-αs1-casein
The environment of the fusion sites is given as sequences. The eight amino acids at the N-terminus and the two amino acids at the C-terminus that were added to <t>CSN1S1</t> due to the cloning procedure are shown in italics. Restriction sites used for cloning are underlined. SP, signal peptide.
Small Interference Rna Si αs1 Casein, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/small interference rna si-αs1-casein/product/Shanghai GenePharma
Average 90 stars, based on 1 article reviews
small interference rna si-αs1-casein - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


The environment of the fusion sites is given as sequences. The eight amino acids at the N-terminus and the two amino acids at the C-terminus that were added to CSN1S1 due to the cloning procedure are shown in italics. Restriction sites used for cloning are underlined. SP, signal peptide.

Journal: PLoS ONE

Article Title: Autoantibodies to αS1-Casein Are Induced by Breast-Feeding

doi: 10.1371/journal.pone.0032716

Figure Lengend Snippet: The environment of the fusion sites is given as sequences. The eight amino acids at the N-terminus and the two amino acids at the C-terminus that were added to CSN1S1 due to the cloning procedure are shown in italics. Restriction sites used for cloning are underlined. SP, signal peptide.

Article Snippet: Goat anti-human IgG conjugated with horseradish peroxidase was obtained from Beckman Coulter (Krefeld, Germany), rabbit anti-human αS1-casein (CSN1S1) was obtained from ModiQuest (Nijmegen, The Netherlands), goat anti-rabbit IgG conjugated with horseradish peroxidase was obtained from Sigma-Aldrich (product number A0545 Munich, Germany) and goat anti-rabbit IgG conjugated with FITC was obtained from Bethly (Montgomery, USA).

Techniques: Cloning

2.1) SDS-PAGE of outer membrane protein preparations from E. coli UT5600(DE3) pKP010 which were incubated without trypsin (lane 1) and cells which were treated with trypsin (lane 2). OmpF/C and OmpA are natural outer membrane proteins of E. coli , and can be used as reporters for successful outer membrane protein preparations. 2.2) Flow cytometer analysis of E. coli cells displaying αS1-casein. (A) E. coli UT5600(DE3) pKP010 were incubated with a polyclonal rabbit antiserum against CSN1S1 casein and subsequently with a secondary antibody conjugated with FITC. (B) The mean fluorescence of E. coli UT5600(DE3) pKP010 was 10,071. The value of the fluorescence for E. coli UT5600(DE3) cells without a plasmid, used as a negative control, was 464. 2.3) Fluorescence microscopy of both cell types. The cells were treated identically as described for flow cytometer analysis (2.2). (A) E. coli UT5600(DE3) pKP010 ; (B) E. coli UT5600(DE3) as negative control; (C) E. coli UT5600(DE3) as negative control, transmission light control.

Journal: PLoS ONE

Article Title: Autoantibodies to αS1-Casein Are Induced by Breast-Feeding

doi: 10.1371/journal.pone.0032716

Figure Lengend Snippet: 2.1) SDS-PAGE of outer membrane protein preparations from E. coli UT5600(DE3) pKP010 which were incubated without trypsin (lane 1) and cells which were treated with trypsin (lane 2). OmpF/C and OmpA are natural outer membrane proteins of E. coli , and can be used as reporters for successful outer membrane protein preparations. 2.2) Flow cytometer analysis of E. coli cells displaying αS1-casein. (A) E. coli UT5600(DE3) pKP010 were incubated with a polyclonal rabbit antiserum against CSN1S1 casein and subsequently with a secondary antibody conjugated with FITC. (B) The mean fluorescence of E. coli UT5600(DE3) pKP010 was 10,071. The value of the fluorescence for E. coli UT5600(DE3) cells without a plasmid, used as a negative control, was 464. 2.3) Fluorescence microscopy of both cell types. The cells were treated identically as described for flow cytometer analysis (2.2). (A) E. coli UT5600(DE3) pKP010 ; (B) E. coli UT5600(DE3) as negative control; (C) E. coli UT5600(DE3) as negative control, transmission light control.

Article Snippet: Goat anti-human IgG conjugated with horseradish peroxidase was obtained from Beckman Coulter (Krefeld, Germany), rabbit anti-human αS1-casein (CSN1S1) was obtained from ModiQuest (Nijmegen, The Netherlands), goat anti-rabbit IgG conjugated with horseradish peroxidase was obtained from Sigma-Aldrich (product number A0545 Munich, Germany) and goat anti-rabbit IgG conjugated with FITC was obtained from Bethly (Montgomery, USA).

Techniques: SDS Page, Membrane, Incubation, Flow Cytometry, Fluorescence, Plasmid Preparation, Negative Control, Microscopy, Transmission Assay, Control

62 sera of healthy volunteers were tested for antibodies against CSN1S1 with the SD-ELISA. Every serum was measured three times independently, the average was calculated and is shown as a column. SD of each mean is given as a line.

Journal: PLoS ONE

Article Title: Autoantibodies to αS1-Casein Are Induced by Breast-Feeding

doi: 10.1371/journal.pone.0032716

Figure Lengend Snippet: 62 sera of healthy volunteers were tested for antibodies against CSN1S1 with the SD-ELISA. Every serum was measured three times independently, the average was calculated and is shown as a column. SD of each mean is given as a line.

Article Snippet: Goat anti-human IgG conjugated with horseradish peroxidase was obtained from Beckman Coulter (Krefeld, Germany), rabbit anti-human αS1-casein (CSN1S1) was obtained from ModiQuest (Nijmegen, The Netherlands), goat anti-rabbit IgG conjugated with horseradish peroxidase was obtained from Sigma-Aldrich (product number A0545 Munich, Germany) and goat anti-rabbit IgG conjugated with FITC was obtained from Bethly (Montgomery, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Eight probands who were breast fed for a short period of time (5 d–42 d), showed an increased antibody reaction against CSN1S1 in comparison to control persons, who were not breast-fed. (dotted line = cut-off value from ).

Journal: PLoS ONE

Article Title: Autoantibodies to αS1-Casein Are Induced by Breast-Feeding

doi: 10.1371/journal.pone.0032716

Figure Lengend Snippet: Eight probands who were breast fed for a short period of time (5 d–42 d), showed an increased antibody reaction against CSN1S1 in comparison to control persons, who were not breast-fed. (dotted line = cut-off value from ).

Article Snippet: Goat anti-human IgG conjugated with horseradish peroxidase was obtained from Beckman Coulter (Krefeld, Germany), rabbit anti-human αS1-casein (CSN1S1) was obtained from ModiQuest (Nijmegen, The Netherlands), goat anti-rabbit IgG conjugated with horseradish peroxidase was obtained from Sigma-Aldrich (product number A0545 Munich, Germany) and goat anti-rabbit IgG conjugated with FITC was obtained from Bethly (Montgomery, USA).

Techniques: Comparison, Control

(A) Antibody reaction against E. coli UT5600(DE3) pSH3 (control, lane1), E. coli UT5600(DE3) displaying human CSN1S1 (lane 2) and E. coli UT5600(DE3) displaying bovine CSN1S1 (lane3) measured by SD-ELISA with a rabbit polyclonal anti- bovine CSN1S1 antibody. For detection a goat HRP conjugated anti rabbit antibody was used according to the conditions described for . (B) The antibody reaction against E. coli UT5600(DE3) pSH3 (control, black columns) and E. coli UT5600(DE3) displaying bovine CSN1S1 (white columns) were analyzed at different concentrations of the rabbit polyclonal anti-bovine CSN1S1 antiserum or in PBS with 3% FCS as negative control. (C) The SD ELISA against bovine CSN1S1 was repeated three times independently in triplicates at the highest antibody concentration applied (200 ng/ml) with E. coli UT5600(DE3)SH3 (control, lanes 1–3) and E. coli UT5600(DE3) displaying bovine CSN1S1 (lane4–6) in order to test the reproducibility.

Journal: PLoS ONE

Article Title: Autoantibodies to αS1-Casein Are Induced by Breast-Feeding

doi: 10.1371/journal.pone.0032716

Figure Lengend Snippet: (A) Antibody reaction against E. coli UT5600(DE3) pSH3 (control, lane1), E. coli UT5600(DE3) displaying human CSN1S1 (lane 2) and E. coli UT5600(DE3) displaying bovine CSN1S1 (lane3) measured by SD-ELISA with a rabbit polyclonal anti- bovine CSN1S1 antibody. For detection a goat HRP conjugated anti rabbit antibody was used according to the conditions described for . (B) The antibody reaction against E. coli UT5600(DE3) pSH3 (control, black columns) and E. coli UT5600(DE3) displaying bovine CSN1S1 (white columns) were analyzed at different concentrations of the rabbit polyclonal anti-bovine CSN1S1 antiserum or in PBS with 3% FCS as negative control. (C) The SD ELISA against bovine CSN1S1 was repeated three times independently in triplicates at the highest antibody concentration applied (200 ng/ml) with E. coli UT5600(DE3)SH3 (control, lanes 1–3) and E. coli UT5600(DE3) displaying bovine CSN1S1 (lane4–6) in order to test the reproducibility.

Article Snippet: Goat anti-human IgG conjugated with horseradish peroxidase was obtained from Beckman Coulter (Krefeld, Germany), rabbit anti-human αS1-casein (CSN1S1) was obtained from ModiQuest (Nijmegen, The Netherlands), goat anti-rabbit IgG conjugated with horseradish peroxidase was obtained from Sigma-Aldrich (product number A0545 Munich, Germany) and goat anti-rabbit IgG conjugated with FITC was obtained from Bethly (Montgomery, USA).

Techniques: Control, Enzyme-linked Immunosorbent Assay, Negative Control, Concentration Assay

61 of the 62 sera shown in with the IgG reaction on human CSN1S1 were analyzed for an IgM mediated serum response on the same antigen by SD-ELISA. Each serum was tested three times, the mean was calculated and shown as columns. SD of each mean is given as a line.

Journal: PLoS ONE

Article Title: Autoantibodies to αS1-Casein Are Induced by Breast-Feeding

doi: 10.1371/journal.pone.0032716

Figure Lengend Snippet: 61 of the 62 sera shown in with the IgG reaction on human CSN1S1 were analyzed for an IgM mediated serum response on the same antigen by SD-ELISA. Each serum was tested three times, the mean was calculated and shown as columns. SD of each mean is given as a line.

Article Snippet: Goat anti-human IgG conjugated with horseradish peroxidase was obtained from Beckman Coulter (Krefeld, Germany), rabbit anti-human αS1-casein (CSN1S1) was obtained from ModiQuest (Nijmegen, The Netherlands), goat anti-rabbit IgG conjugated with horseradish peroxidase was obtained from Sigma-Aldrich (product number A0545 Munich, Germany) and goat anti-rabbit IgG conjugated with FITC was obtained from Bethly (Montgomery, USA).

Techniques: Enzyme-linked Immunosorbent Assay